Specifications include, but are not limited to: Determine the chronic and/or acute toxicity of final effluent from the City's Walnut Creek and South Austin Regional Wastewater Treatment Facilities; the City's Austin Energy Sandhill and Decker Power Plants; and the effluent receiving streams of these facilities, on the freshwater organisms as described below: 2.1. Method 1002.0, Ceriodaphnia dubia 7-day survival and Reproduction Test: 2.1.1. Minimum Frequency, Wastewater Facilities: 8/year for wastewater (2 facilities quarterly). 2.1.2. Minimum Frequency, Power Plants: 8/year for power plants (2 plants quarterly). Testing may become less frequent (semi-annually) as permit dictates. 2.2. Method 1000.0 Pimephales promelas (Fathead Minnow) 7-day Larval Survival and Growth Test: 2.2.1. Minimum Frequency, Wastewater Facilities: 8/year for wastewater (2 facilities quarterly). 2.2.2. Minimum Frequency, Power Plants: 8/year for power plants (2 plants quarterly). Testing may become less frequent (semi-annually) as permit dictates. 2.3. Acute 24-hour static toxicity test using Daphnia pulex: 2.3.1. Minimum Frequency, Wastewater Facilities: 4/year for wastewater (2 facilities semi-annually). 2.3.2. Minimum Frequency, Power Plants: 8/year for power plants (2 plants quarterly). Testing may become less frequent (semi-annually) as permit dictates.