Specifications include, but are not limited to: IVIS Spectrum Imaging System The IVIS Spectrum provides a highly sensitive in vivo, ex vivo and in vitro 2D and 3D imaging system that is user-friendly for both bioluminescent and fluorescent detection. The IVIS Spectrum is intuitively designed with the modern researcher in mind by providing unique and innovative accessories to improve workflow and efficiency. The system includes a highly sensitive, cooled CCD camera, light-tight imaging chamber and complete automation and analysis capabilities. The IVIS Spectrum Imaging System is a high-sensitivity, low noise, in vivo imaging technology platform that enables noninvasive visualization and tracking of cellular and genetic activity within a living organism in real time. The system provides both bioluminescence and fluorescence imaging capability. For fluorescence imaging, the instrument can operate in reflectance or transillumination mode. Filtered light from a broad-band lamp provides the excitation source in both modes. In the reflectance mode, light is delivered to four reflectors that are located on the ceiling of the imaging chamber. In the transillumination mode, the excitation light is delivered to an x-y translation assembly under the stage and focused to a 2 mm diameter beam that can be directed to a particular location on the underside of the animal subject. The system includes ten excitation and 18 emission filters that enable spectral scanning of reporters over the range from 480-850 nm. The system also includes a structured light projector that enables reconstruction of the surface topography. The 3D location and concentration of fluorescent sources can be computed from structured light and transillumination fluorescent images. The 3D location and strength of luminescent sources is computed from structured light and luminescent image data. Features and Benefits: CCD Camera Specification Sensor Type Back illuminated CCD Format 2048 x 2048 pixels CCD Size 26 x 26 mm Effective CCD Format 1920 x 1920 pixels Pixel Dimensions 13.5 x 13.5 μm Quantum Efficiency ~85% 400-700nm >50% 350-900nm Readout Noise <5 electrons Dark Current RMS <100 electrons/s/cm2 (-90° C) Minimum Detectable Luminance <70 photons/s/sr/cm2 CCD Temperature Nominal -90° C Optical Component Specification Lens f/stop f/1- f/8 Field of View (FOV) 3.9, 6.5, 13, 22 cm Resolution >60 μm (FOV = 3.9, f/1) Scanning Laser <1 mW at 532 nm Fluorescence Imaging Component Specification Excitation Filters 12 positions, 25 mm diameter, 10 filters supplied Emission Filters Background (Autofluorescence and Leakage) 22 positions, 60 mm diameter, 18 filters supplied Background: 1 part in 10^7 typical Lamp 150 W quartz halogen, 21V, 3250° Kelvin Supports Spectral Unmixing using proprietary Compute Pure Spectrum (CPS) algorithms to ensure accurate separation of mixed fluorescent components and thus individual fluorophore quantification Software controlled excitation/emission filter selection for turnkey operation Environmental Component Temperature Specification Temperature: 15 -25° C (59-78° F) Humidity 0-80% non-condensing Type of Use Indoor Sound Level 61 dB < 500 Hz Stage Temperature Ambient to 40° C Altitude Rating <2000 meters (6560 ft.)
