This document provides specifications for a requirement contract for laboratory services for the analysis of protozoa and other biological species, which the City of Cleveland (City), Division of Water (CWD) may require to be performed. CWD intends to use this specification and the resulting requirement contract to laboratory services for protozoa and biological analysis for a period of two (2) years. provide This standard is for the determination of biological species including, but not limited to, the following: A. Protozoa, primarily Cryptosporidium and Giardia, by United States Environmental Protection Agency (USEPA) Method 1623.1. USEPA Method 1623.1 is a performancebased method for the determination of Cryptosporidium and Giardia in aqueous matrices. The method requires filtration, immunomagnetic separation of the Cryptosporidium oocysts and Giardia cysts from the material captured by a filter, and an immunofluorescence assay for the determination of Cryptosporidium oocyst and Giardia cyst concentrations, with confirmation through vital dye staining and differential interference contrast microscopy. В. Legionella, by Centers for Disease Control (CDC) Method: Procedures for Recovery of Legionella from the Environment. the C. Microscopic Particulate Analysis, by USEPA's Consensus Method for Determining Groundwaters under the Direct Influence of Surface Water Using Microscopic Particulate Analysis (MPA).
