Work Area: The BSL-3 work area shall include Rooms 2206 (Main Lab), three (3) Isolation Suites (approximately 150 sq. ft – each suite), Autoclave Room, Shower Room, anteroom, and adjacent hallways and rooms. Total work area: 1950 square feet. Schedule: Work shall be completed within two (2) months of Notice to Proceed. Equipment: Bidder shall provide all equipment required to perform the necessary tests. All measurement equipment used shall be NIST traceable and copies of the NIST calibration certificates shall be included in the final report. Verification of Air Flow, Temperature, and Humidity: Differential pressure testing and smoke pencil visualization between rooms to verify directional airflow is drawn into the BSL-3 laboratory from "clean" areas and move towards “less clean” area of the suite. Functional testing of the HVAC systems and visualization of room airflow patterns and containment using smoke to verify that sustained positive pressurization of the laboratory is prevented. Containment will be verified during normal operating conditions, no power, transition periods, emergency generator back-up power conditions, and return to normal operation. All actual supply and exhaust airflows will be measured in the suites, recorded, and compared to the suite design air exchange rates. Measure the air flow at supply and exhaust registers using NIST traceable equipment and compare the suite design air exchange rates. Measurement of temperature and humidity from various locations in the laboratory space. Verification will include ten-day stability measurements. Verification of local and remote alarms. i.e., suite negative pressurization failure (if applicable). Preparation of Report Report shall contain the following: Present raw data in both table and graphical formats. Analysis of data to determine the suitability of the air flow in the laboratory area for use for BSL-3 and BSL-2 work following the recommendations found in the BMBL-6. Present conclusions regarding the suitability of the spaces for use. Provide recommendations to address any issues. Provide a list of all equipment used for testing and copies of the calibration documentation which covers the period in which the equipment was used.
