Product Requirements a. Technical Specifications (Minimum Requirements): Sequencing Technology: Sequencing-by-synthesis (SBS) or equivalent Read Length: 2 × 150 bp paired-end reads minimum Throughput per Run: At least 5 million reads (25 million or more preferred) Flow Cell Type: Single-use cartridge or flow cell with onboard clustering Data Output: ≥ 1.2 Gb per run (minimum), ≥ 7.5 Gb preferred Run Time: Complete workflow (from load to FASTQ) in ≤ 24 hours Base Calling Accuracy: ≥ Q30 for ≥75% of bases Sample Multiplexing: Must support indexing and multiplexing (≥ 96 samples per run); b. Performance Expectations: Capable of generating high-quality amplicon sequencing (or targeted gene sequencing) and small genome assemblies. Suitable for transcriptomics, microbial genomics, targeted gene sequencing, and pathogen detection and surveillance. Compatible with low- and mid-throughput research and diagnostic workflows; b. Compatibility Requirements: Must be compatible with existing library preparation kits, including Nextera XT, 16S metagenomics, and amplicon-based workflows Compatible with standard Illumina or equivalent adapter/indexing systems Capable of working with existing analysis tools (BaseSpace, Galaxy, or similar); c. Quality Standards / Certifications: ISO 13485 or ISO 9001 certified manufacturing (or equivalent)
