• Components: Each kit must include 96-well plates and all necessary reagents. • Instructions: A detailed assay procedure must be provided with the kit. • Sample Compatibility: Kits must be suitable for use with blood, plasma, serum, and urine. • Testing Accuracy: o True Positive: Positive results must be accurate. o False Negatives: The test must accurately detect the presence of the substance. o True Negative: Negative results must be accurate. o False Positives: Minimize incorrect positive results when the substance is not present. • Cross-Reactivity: Provide a summary of cross-reactivity with structurally related compounds in blood and urine. • Robotic System Compatibility: The kit must include a script compatible with the TECAN Freedom EVO 75 robotic pipetting system, using the following software: o Freedom Evoware 2.8 Service Pack 3 o Navitrax 2 o Magellan 7.2 Specific Kits Required: • Amphetamines: o Target: S (+)-Amphetamine o Cutoff: 20 µg/L • Methamphetamines: o Target: S (+)-Methamphetamine o Cutoff: 20 µg/L o High cross-reactivity with D-methamphetamine and low cross-reactivity with L-methamphetamine • Benzodiazepines: o Target: 7-aminoclonazepam o Cutoff: 10 µg/L • Cocaine Metabolite: o Target: Benzoylecgonine o Cutoff: 50 µg/L • Opiates: o Morphine: Cutoff at 10 µg/L (previously 20 µg/L) o Oxycodone: Cutoff at 50 µg/L • Cannabinoids: o Target: THCA o Cutoff: 10 µg/L • Oxycodone: o Cutoff: 10 µg/L • Ketamine: o Cutoff: 10 µg/L • Zolpidem: o Cutoff: 10 µg/L (previously 25 µg/L) • Fentanyl: o Cutoff: 1 µg/L (previously 5 µg/L) • Buprenorphine: o Cutoff: 1 µg/L
