Specifications include, but are not limited to: Multiphoton Customized (MPC) Microscope Optimized for 2-photon and 3-photon imaging from 680nm to 1300nm and 1700nm *Upright configuration Key features: Large space under the objective. Super-large 2" fluorescence collection optics, achromatically compensated, enabling deep 3D & low-power imaging thus minimizing photo-bleaching and photo-toxicity. Beam size up to a Ø23 mm at back aperture objective for highest resolution multiphoton Imaging. Free space laser input port. 3 position motorized and software controlled objective turret, M32 x 075 threaded. Motorized and software controlled modality change. Integrated workspace illumination for easy sample navigation and monitoring. Supports a wide range of imaging modalities, like epi-fluorescence widefield, 2P, 3P, 4P, TD-FLIM, FD-FLIM, CARS, SRS. 2 pcs. high sensitive GaAsP PMTs with transimpedance amplifiers. Transimpedance amplifiers gain and bandwidth software controlled Galvo - Galvo type scan engine Mechanical interface supporting 60mm cage system for home-built add-ons in external laser beam line US keyboard, mouse, monitor, built-in high performane PC systemScanImage, Chromogazer, ImageJ Environmental specifications: Air-cooled (might not apply for ordered laser system); single phase, 110V - 230V autoswitching, 20 A max; operating environment 18°C - 28°C; storage temperature -15°C to +50°C; humidity 10% - 90% (non condensing); noise level <70 dBa; altitude 2500 m max. System includes: 2 PMTs non-descanned epi direction - Hamamatsu H10770PA-40 2 PMT fluorescence filter cubes - Two filter cubes defined at the time of purchase *0 Objective - Objective supplied by user; system will be tested and manufacture certified with Olympus - N20X-PFH 1 Galvo - galvo scan system - 1.6 fps at 512 x 512 Pixels, 0.1 fps at 2048 x 2048 Pixels, Pixel dwell time: 0.8 to 32 microseconds. Field of view (FOV): >20 mm diagonal square (Max) at the intermediate image plane. Typically 0.7 mm x 0.7 mm by using the Nikon 20x objective. Beam diameter @ objective back aperture plane up to 23 mm 1 Software - Chromogazer (by Prospective Instruments) microscope control, diagnostics, modality change, etc. - fully script capable, source code available on request MBF / Vidrio ScanImage Premium MATLAB ImageJ Microsoft Windows 11 64-bit 1 PC & display ATX gaming mainboard, AMD Ryzen 9 3900X, 32 GB RAM DDR4, 500 GB SSD, Graphics card. Display 27“; Keyboard (QWERTY), mouse; Widefield epi fluorescence imaging Widefield single photon fluorescence imaging includes: Illumination source (white light) Fully integrated white light illumination engine Spectral coverage 385 nm - 730 nm Output power >3W White light collimator; LaserCron3P Stand-alone ultrafast laser for 3P, 4P, 5P, SHG & THG (works also for 2P@1250nm): Tuning range: 1250 - 1800 nm Repetition rate: Single-shot to 1 MHz / 2 MHz Pulse duration: < 50 fs@1300 nm, < 65 fs@1700 nm Output power @ 1300nm: > 1100 mW @ 1 MHz, > 800 mW @ 2 MHz Output power @ 1700nm: > 800 mW @ 1 MHz, > 500 mW @ 2 MHz Continuous GDD control range@1300nm: -4000 to +9000 fs2 Continuous GDD control range@1700nm: -12000 to +3500 fs2 Beam quality (M2) < 1.2 Beam ellipticity > 0.8 Long-term power stability, 24 h: < 1% Pulse-to-pulse energy stability: < 1% Operating temperature: 19 - 25 ºC (air conditioning recommended) Relative humidity: 20 - 70% (non-condensing) Electrical requirements: Laser 100V AC, 7A - 240V AC, 3A; 50 - 60 Hz Chiller 100 - 230V AC; 50 - 60 Hz