Please note: This is a Sole Source Notification. Specifications include, but are not limited to: Our foremost criteria for choosing a solid phase extraction (SPE) handling system is the integration of a positive pressure system for solvent elution. Solid phase extraction cartridges are analogous to a syringe filled with a sorbent used for separating classes of compounds by affinity, polarity, size or another physical characteristic. These columns come in several formats, individually as a cartridge or as multiwell plates. There are three methods to process solvents through these columns, gravity, vacuum, or pressure. Gravity is the simplest procedure but is slow, prone to clogging, and often incompatible with viscous samples or solvents. To assist the movement of liquid through a column, force is applied, either as a vacuum from the bottom of the well or as positive pressure from the top. Historically, the negative pressure manifold predominated, it was simple to implement when processing samples individually in the cartridge format. With the adoption of high throughput devices that use multiwell plates, unused wells need to be blocked to prevent the applied vacuum being pulled primarily through empty wells. In addition, the vacuum is difficult to regulate, with some wells inevitably running faster than others which again, leads to empty wells and the vacuum over drying faster eluting wells resulting in uneven recovery. To solve the shortcomings of vacuum extraction, positive pressure devices have been developed and marketed. These are simple devices, after the addition of solvent to the cartridge, a pressure is applied by flowing a gas (air or nitrogen) from the top, to push the solvent through. The positive pressure device offers several advantages, 1) it does not suffer from failure if a well runs dry or needs to have empty wells blocked, 2) it has a higher-pressure range and is able to process more viscous samples, and 3) the pressure is much easier to regulate, allowing for flow rate optimization. The Tecan Resolvex is capable of operating as a self-contained unit and can perform up to 11 wash and elution steps within the device itself. In short, the Tecan Resolvex A200 is the only system that meets the needs of the Metabolomics Core. It has two unique features that no other commercially available system has. It uses positive pressure to elute solvent from the SPE cartridges and is a standalone unit.