Specifications include, but are not limited to: The Alaska Department of Fish and Game Gene Conservation Laboratory (ADF&G – GCL) is soliciting bids forchemicals and plastic consumables for single nucleotide polymorphism (SNP) genotyping assays and for shorttandem repeat (STR) PCR via capillary electrophoresis (CE) to be used on Pacific salmon, arctic shellfish, geoduck clam, sea cucumber, Pacific seaweed, and bear DNA. The items will be for a combination of SNP genotyping andSTR PCR via CE.1.A PCR chemistry master mix;2.Oligonucleotide primer/probe sets;3.A passive presence/absence reference dye;4.A dNTP blend;5.PCR primer pairs;6.CE size standard for PCR product between 50 and 500 base pair length;7.CE size standard for PCR product between 50 and 600 base pair length;8.CE running buffer;9.CE polymer;10.Formamide;11.A DNA Normalization Plate Kit;12.A CE capillary array;13.CE instrument water, waste, and buffer reservoirs and reservoir caps;14.CE instrument water, waste, and buffer retainers;15.CE instrument water and waste bases;16.CE instrument cathode buffer plate base;17.CE instrument water, waste, and buffer septa;18.CE sample septa;19.CE instrument ferule sleeve;20.A 384-Well PCR Plate and;21.CE instrument anode buffer jarAlthough ADF&G – GCL cannot guarantee a minimum quantity of purchase, based on projections, ADF&G – GCLanticipates needing enough chemicals to run approximately 192 SNP assays for 60,000 – 100,000 fish in 5µLreaction volumes, and 70 STR markers for 20,000 fish in 10µL reaction volumes per year.
