Specifications include, but are not limited to: The CONTRACTOR must provide the services of one (1) Microbiologist with the following knowledge, skills and abilities at the time of hiring: Working knowledge of scientific inquiry, isolation, identification and characterization of microorganisms. Knowledge of the relationship between microorganisms and the disease. Working knowledge of real-time Polymerase Chain Reaction (PCR) and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) General knowledge and experience in application and issues involving molecular diagnostic assays of bacterial and viral diseases General knowledge of nucleic acid sequencing and analysis. Working knowledge of Laboratory Information Management System (LIMS). Working knowledge of scientific equipment and supplies, personal protective equipment (PPE) and Biosafety Level 2 and 3 Laboratory practices. Ability to conduct independent analysis, with minimum guidance, for the culture and isolation of pathogens from various materials, including clinical, non-clinical and environmental samples. Ability to perform varied and highly specialized microbiological and molecular assays for the detection and identification of various agents as well as new and re-emerging infectious disease agents. Ability to maintain aseptic techniques in media and reagent preparation following standard laboratory techniques and practices. Ability to operate and maintain standard and specialized laboratory equipment. Ability to determine independently the proper protocol/procedure needed for analysis. Ability to interact and communicate with peers. Ability to follow oral and written instructions and ability to communicate in written and verbal methods. Skilled in the performance of real-time Polymerase Chain Reaction (PCR) and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Skilled in performing nucleic acid extraction (manual or using semi-automated or automated platforms) from clinical specimens/isolates or environmental samples.