Specifications include, but are not limited to: Purchase of a fluorescence microscope with live cell imaging capability.; Suitable for live cell imaging, detection of protein-protein interactions by FRET and colocalization, and 2D and 3D analysis of cells, spheroids, and organoids.; Microscope must: be fully automated; have extra ports for future upgrades or addition of third-party components; have drift correction and fast focusing for long-time live cell imaging; be able to hold well plates, petri dishes, and glass slides; have a motorized scanning stage; come with an on-stage incubator capable of temperature and gas controls; Fluorescence light source must: contain at least four separate LEDs covering classical dyes and FPs; come with filter cubes covering classical dyes and FPs as well as allowing to perform FRET; Image capture system must: be better than a Hamamatsu Orca-Fusion sCMOS camera; consist of a cooled sCMOS, monochrome fluorescence camera with high quantum efficiency and low read/dark noise; offer high speed, high resolution (at least 20482048), and extended dynamic range with pixel size of 6.5 mm and sensor diagonal of 18.8 mm.; include a high-quality color camera as an optional item; Objective lens must include: N plan achromatic 5x/0.1; plan FLUOTAR 10x/0.3, 20x/0.4, 40x/0.6; plan apochromatic 40x/0.95; plan apochromatic 63x/1.4-0.6 oil; immersion oil; lens cleaning spray bottle and cleaning solution