Specifications include, but are not limited to: The Contractor(s) shall: A. Provide analysis in an indefinite quantity for the determination and detection of microbiological contaminants in accordance with the SDWA: 1) the presence or absence of Total Coliform and Escherichia (E.) coli as specified in the GWR (40 CFR 141.402(c)) or the RTCR (40 CFR 141.852); and 2) the enumeration of E. coli as specified in the SWTR (40 CFR 141.74(a)). 1) GWR: (40 CFR 141.402(6c). DWB has determined that E. coli will be the fecal indicator used for GWR compliance. 2) RTCR: (40 CFR 141.852) Analytical methodology (a) The standard sample volume required for analysis, regardless of analytical method used, is 100 mL. (b) Systems need only determine the presence or absence of total coliforms and E. coli; a determination of density is not required. (c) The time from sample collection to initiation of test medium incubation may not exceed 30 hours. Systems are encouraged but not required to hold samples below 10 deg. C during transit. d) If water having residual chlorine (measured as free, combined, or total chlorine) is to be analyzed, sufficient sodium thiosulfate (Na2S2O3) must be added to the sample bottle before sterilization to neutralize any residual chlorine in the water sample. Dechlorination procedures are addressed in Section 9060A.2 of Standard Methods for the Examination of Water and Wastewater (20th ed., 21st ed., 22nd ed., and 23rd ed.). (e) Systems must conduct total coliform and E. coli analyses in accordance with one of the analytical methods in the following table. 3) SWTR: (Methods cited from (40 CFR 141.74(a)).