Specifications include, but are not limited to: 1. Point scanning confocal microscope. 2. STED and FLIM capabilities. 3. Transmitted light capabilities with differential interference contrast. 4. Confocal fluorescence excitation at multiple laser lines 405 nm, 488 nm, 561 nm, and 640 nm, or equivalents (+ 10 nm). 5. 1 or 2 STED lasers (592nm and/or 775nm). 6. 4-color LED wide-field excitation and corresponding excitation and emission filters for fluorescence visualization through the eyepieces. 7. LED or halogen illumination for brightfield visualization through the eyepieces. 8. Multiple objective magnifications that fill the range of 10x to 63x magnification. 9. 2 to 4 high sensitive detectors for fluorescence and FLIM, and one detector for brightfield/DIC. 10. Z-piezoelectric stage. 11. PC workstation, monitor, and software to control the microscope.