Specifications include, but are not limited to: • The mass spectrometer must have dimensions of no more than 1270 × 767 × 703 mm (w, d, h). • The mass spectrometer must consist of three mass analyzers, the first built on quadrupole technology, the second built on linear ion trap technology, the third on Orbitrap technology. Each mass analyzer must be able to work sequentially with the others, or in parallel with the others. • The mass spectrometer must have two independent mass spectrometers, one based on linear ion trap technology and the other on Orbitrap technology. The mass spectrometers must operate independent of the other or in concert with the other. • All three mass analyzers must be capable of real-time mass analysis and Orbitrap and Ion trap must be capable of real-time ion detection and data processing, enabling a high spectrum generation rate. The operation of the three mass analyzers must be underDynamic Scan Management for operational efficiency. • The mass spectrometer must be capable of Real-Time Search of a protein database to identify peptide spectra on-the-fly and to intelligently direct MSn data acquisition. • The mass spectrometer must be under an Xcalibur based data system for instrument control and acquisition. • The mass spectrometer must be able to perform parallel detection; within one minute of analysis time it should be able to acquire at least 30 full spectra at a resolution of 120,000 @ m/z 200 using the Orbitrap detector and 1200 data dependent MS/MS scans of the parent ions isolated with quadrupole, fragmented in the Ion Routing Multipole and detected with the ion trap detector.
