Specifications include, but are not limited to: Stage-top Incubation system*to be used on a confocal microscope stage•Ability to be used with premixed 5% CO2 or with the purchase of a gas mixer, in which case 100% can be used.•Must be user-friendly and easy to use without cumbersome cables•Easy and quick to change between different types of imaging vessels.•Expandable to accommodate future needs and add-ons.•Responsive and knowledgeable support staff•Must contain several custom chambers for a variety of purposes as well as incubators specifically for microfluidic experiments.•Single incubator that is capable of holding 96, 48, 24, 12, 6 well plates, 35mm dishes, 55mm dishes, chambered slides, chambered coverglasses•Include two magnetically sealing 35mm format re-useable imaging chambers•Heats both from the top and the bottom•Perfusion-ready with existing inlets/outlet ports•Include a glass imaging cover for standard, plastic, disposal 35mm dishes•External humidifier to control evaporation of media •Lens warmers•Must fit scanning stage 130x100 STP set for Zeiss LSM