A science-based WVC reduction and landscape connectivity improvement program in NYS is urgently necessary due to the risks that wildlife vehicle collisions (WVCs) pose to human life and safety and to wildlife population viability (including projected species range shifts from climate change), and the cost burden WVCs inflict via property damage and maintenance and operational expenses. NYSDOT has pursued opportunities to improve connectivity and integrate wildlife passage into multiple projects. However, systematic inclusion in NYSDOT’s capital program has been challenging due to incomplete information regarding WVC priorities, connectivity improvements, and barrier mitigation. NYSDOT is seeking to address these knowledge deficiencies by developing a data-driven Toolkit that informs decisionmaking with quantifiable evidence. Ultimately, the Toolkit is the first step toward achieving WVC reduction and connectivity improvement programs throughout New York State’s roadways. The Toolkit will be used via a browser-based GIS application assembled by NYS Office of Information and Technology Services (OITS) using NYSDOT’s Esri platform. All files shall be submitted as Esri File Geodatabases. The Toolkit must provide the following information: * Identification of priority areas where WVCs are occurring at the greatest rate and severity, relative to the rest of the state. * Determination of where roads impede aquatic and terrestrial connectivity. * Prioritization of opportunities to reduce WVCs and improve habitat connectivity. * Integration of additional ecological, economic, cultural, equity, and demographic data to produce a robust model for decision making and prioritization of sites. * Identification of communities in close proximity to these priority areas where targeted education and outreach may be beneficial.