The time-lapse, in vitro incubator microscope system should be fully integrated to allow for uninterrupted embryo monitoring. Gas Atmosphere: System must support tri-gas incubation with precise control of temperature, humidity, and gas composition (CO2 and O2 regulation, balance with N2). Culture Capacity: Ability to simultaneously culture and image at least 230 embryos using individual wells of separate disposable/consumable culture dishes (custom dishes is fine) to minimize cross-contamination and mixing. Imaging: Automated brightfield microscopy with multi-plane z-stack image acquisition at defined intervals throughout culture. Environmental Stability: Chamber designed for minimal disturbance during imaging, ensuring consistent embryo culture conditions. Data Management: Dedicated workstation and software enabling embryo annotation, playback, and comparative analysis of embryo development sequences Must allow continuous culture from fertilization through blastocyst stage Must include installation and user training Package must include all start-up accessories and consumables
