Requested tests: Mysid (Mysidopsis bahia) 7-day, static renewal, chronic toxicity test for survival, growth, and fecundity. EPA Method 1007.0 Inland Silverside (Menidia beryllina) 7-day, static renewal, chronic toxicity test for larval survival and growth. EPA Method 1006.0 This special project is designed to provide lethal and sub-lethal data utilizing a shrimp and fish species in marine samples. These methods shall include the effects of synergistic, antagonistic, and additive effects of all chemical, physical, and additive components which adversely affect the physiological and biochemical functions of the test organisms. The test concentration shall consist of 100% sample water (i.e., no dilutions) and a control. A definitive test consisting of 5 sample dilutions is not necessary. Because this is not a standard NPDES permit compliance test, abbreviated chronic test methods can be incorporated (i.e., less replicates and less organisms per replicate) as long as the data still has statistical significance. It is anticipated that a volume of 4-gallons will be collected for each of the 8 samples and it’s expected to be sufficient volume for all the test solution renewals needed for the 7-day test.
