Specifications include, but are not limited to: The supplied High Performance Liquid Chromatography System must be capable of: Running method 537.1. Reproducibly injecting up to 10-uL aliquots Performing binary linear gradients at a constant flow rate. The supplied mass spectrometer must be capable of: Triple Quadrupole Mass Spectrometer. Capability of auto tune optimization and automatic calibration of system. A vacuum System shall be fully protected and use turbomolecular pumps. Mass range from 5-3000 m/z. Running method 537.1 The supplied Solid Phase Extraction(SPE) must be capable of: Ability to hold 0.5 g, 6-ml SPE cartridges Vacuum Extraction Manifold Sample Delivery System Extract Concentration System