Specifications include, but are not limited to: The Company shall extract DNA, using any validated manual or robotic magnetic bead based DNA extraction method and shall amplify and type using the GlobalFiler and the Applied Biosystems 3130, 3130xL, 3500 or 3500xL Genetic Analyzer. The analysis shall be produced using the GeneMapper ID-X software versions either 1.4 or 1.5. • Based on the review of quantification data obtained by any validated real-time PCR based quantification system that includes a total human DNA quantity value, a male DNA quantity value, an internal positive control, and preferably an indicator of degradation, a sample need not be amplified. In this case, any elimination or victim samples associated with the specific case likewise do not have to be amplified. • Allele sizes and designations shall be determined with an appropriate internal lane standard and allelic ladder which represents all the common alleles for that particular locus. • At least one allelic ladder and at least one positive amplification control must size correctly during electrophoresis for the associated data to be reported.