Specifications include, but are not limited to: A hybrid high resolution, high mass accuracy mass spectrometer coupled with an ultrahigh pressure liquid chromatography system, capable of nanoliter (10-9 L) flow rates, for proteomics applications. 1. The system must contain both the mass spectrometer (MS) and an ultra-high pressure liquid chromatograph (UHPLC). 2. The UHPLC must contain an autosampler. The autosampler should have an injection loop of 20 L with an injection volume range of at least 0.5 – 18.5 L. 3. The injection volume reproducibility should be 0.3% (RSD) at 10 L or better. The flow rate reproducibility should be 1.0% (RSD) at 500 nL/minute or better. The injection volume should have a linear response over the range of 1 – 15 L. Sample carryover shall be less than 0.003% or <30 parts per million. 4. The autosampler needs to be capable of handling either vials (minimum of 54 vials) or microtiter plates (96 well format) and the autosampler needs to be capable of cooling the samples to 7C, 14C, or holding at room temperature. 5. The pumps for the UHPLC need to be capable of adjusting the flow rates between 50 – 2000 nL/min in a gradient mode. The pumps should have a ceramic piston and capable of pressure driven sample loading. The pumps shall have an upper pressure limit of at least 1000 bar. 6. The UHPLC system should have four 6-port, multi-position UHPLC valves. 7. The UHPLC shall include a column heater that is adjustable between room temperature and at least 50C and connect to the mass spectrometer ion source with zero dead volume (direct connection). 8. The mass spectrometer portion of the integrated system shall be a hybrid instrument designed for MS/MS analyses of proteins and peptides. 9. The mass spectrometer should have a mass accuracy in the MS and MS/MS as follows: with internal calibrant – better than 800 parts per billion RMS error; with external calibrant – better than 2 parts per million RMS error. 10. A single calibration of the mass spectrometer will be valid for both MS and MS/MS analysis and the calibration is independent from charge state of the calibrant mass. 11. The mass drift of the mass spectrometer over 8 hours (with a less than one degree temperature change) shall be less than 2 parts per million. 12. The mass resolution of the mass spectrometer shall be at least 50,000 (full sensitivity) at a mass-to-charge ratio of 1222. 13. The full scan sensitivity of the mass spectrometer in the MS mode shall be as follows: In the electrospray ionization mode: using 1 picogram of reserpine on column, the signal-to-noise ratio shall be at least 100:1 RMS. Computer Specifications (minimum): Windows 7 Pro or 10 (preferred): 64bit operating system 16GB DDR4-2133 (memory) 1GB graphics card 2TB 7200 RPM SATA Hard drive Keyboard Optical 3-Button Mouse Blu-ray Writer Ethernet I210-T1 PCIe NIC (or comparable) Two 21” (minimum) LCD monitors