Specifications include, but are not limited to: A Laser Scanning Confocal microscope is the only instrument capable of the broad range of fluorescent detection in living systems, while maintaining a narrow optical section. The faculty in sum have several needs that are uniquely met by the quoted Leica SP8 confocal microscope. These include 1) having the ability to utilize a very broad number of dyes and fluorescent molecules; multi-spectral excitation, 2) being able to precisely select the detection wavelength in order to truly empower simultaneous multi-spectral imaging, e.g., allow us to image multiple dyes at the same time that have relatively small differences in the wavelength of the emitted light, 3) that have a scan head capable of extremely fast scanning modes for rapid collection of imaging data and super sensitive sensors capable of detecting weak light signals, 4) have a large field of view (FOV) for collection images from high definition images from larger samples, 5) a highly precise automated z-galvo movement to collect ultra-thin section, 6) the scope must be fully capable of differential interference contrast (DIC) to image transparent tissues.