Specifications include, but are not limited to: Surveillance is a non-chemical inspection method that involves classification of mosquito breeding sites, larval presence and distribution surveys, and adult mosquito biting and trapping surveys, as well as identifying mosquitoes to species. Larval surveillance would be conducted while the employee was undertaking larviciding. Adult surveillance should be conducted weekly. If not to be tested for arboviruses at this time, then to be used to measure species composition and population size of adult mosquitoes. This can later be used in addition to larval sampling to determine efficacy of program. Adult surveillance tools to be used on island include CDC light traps. Light and dry ice traps, gravid traps and resting traps. Assume 100 surveillances. Testing During the mosquito season, on a weekly basis, the successful bidder should conduct larval surveys. This means that all sites that have been known to contain mosquitoes in the past are checked for the presence of mosquito larvae. This is accomplished through the use of a standard dipper (350 ml). Other methods of direct inspection include searching for new larval habitats (i.e. artificial containers) and developing mosquito habitats (i.e. water that has been blocked from running). Records of larval stage and numbers per dip would be recorded with monthly reports. A few samples should be saved for later ID. Landing rates of adult mosquitoes are also noted. Further, light traps are placed at various locations around the county. Mosquitoes are removed from the light traps, counted, and identified. Finally, although it can be subjective, discussing the level of the problem with people in the area can help in a pest inspection. Assume 100 tests. Treatment While source reduction is the preferred treatment choice, there are some circumstances where removing standing water would be impractical. In these cases a larvicide is used. The type of larvicide used to control the mosquitoes will depend upon the developmental stage the insects have reached when discovered. Larvae in the 1-3 instar stages are treated with either Bti (a bacteria) or in a catch basin environment Bacillus sphaericus in water soluble pouches. Fourth instar mosquito larvae and pupae must be treated using a different method, as the larvicides already mentioned are not able to affect their development at these stages. A light mineral oil is applied in cases where the mosquitoes have developed past the 3 instar stage. Catch Basin Management: a targeted larviciding treatment to catch basins and storm drains to control mosquitoes in their aquatic stages and prevent emergence as adult mosquitoes especially the suspected West Nile Virus vector Culex (sp.) that typically breeds in these structures. These should be inspected to determine possible mosquito breeding locations. Assume 100 treatments.