Specifications include, but are not limited to:This specification covers Hydrostatic Pressure Chamber requirements for a California Department of Fish and Wildlife (CDFW) program for pressure induced triploidy of Chinook salmon eggs and trout eggs. The Hydrostatic Pressure Chamber will be used to produce triploidy in Chinook salmon eggs and trout eggs. Triploid Chinook salmon eggs and trout eggs will be verified by flow cytometry with results at no less than 95% confidence. The resulting sterile fish will be stocked in inland reservoirs for providing Chinook and trout recreational fisheries in several regions. The chamber shall be equipped with or be capable of the following: 3.1.1 The chamber shall have a minimum internal capacity of 6.0 liters. 3.1.2 The chamber shall be mounted on a moving stainless steel and/or galvanized steel frame. 3.1.3 The chamber frame shall include a wheel and braking system to ensure mobility and stability of the Chamber. 3.1.4 The chamber shall include a winch or lifting device to ensure the safe removal and replacement of the lid to the chamber. 3.1.5 The chamber shall be capable of reaching pressures between 10,000 psi (minimum) and 12,000 psi (maximum). 3.1.6 The chamber shall be equipped to ensure the pressure can be maintained for a minimum of five (5) minutes and allow for pressure adjustments to increase lowering speed. 3.1.7 The chamber shall be equipped with a measuring device (gauge) for monitoring the pressure within the chamber and visibility to the operator. 3.1.8 The chamber shall include a safely release pressure mechanism to relieve pressure at the end of the pressure cycle process. 3.1.9 The chamber shall include a safety device to ensure the chamber cannot be opened while pressurized. 3.1.10 The unit shall be operated using 110-volt electric supply meeting United States standards. 3.1.11 The unit shall include a ≥ 12-foot cord using a flexible tubing application connected to a 110-volt outlet which shall be waterproof to ensure safety from electrical shorting or circuit damage. 3.2 MATERIAL 3.2.1 The chamber shall be made of non-corrosive materials. 3.2.2 All electrical components shall be protected from exposure to water or other liquids that would pose a danger to users, location and equipment. 3.2.3 The chamber shall be designed to allow for mobility of the chamber between several hatcheries throughout the State of California.